The aim of the present study is to evaluate diversity among three Lupinus species (Lupinus albus L., Lupinus angustifolius L., and Lupinus digitatus Forssk.) using seed protein electrophoresis and amino acid composition in order to elucidate the taxonomic relationships among them. In addition, to compare the L. albus var. albus with closely related wild relatives in a trial to attain information useful for breeding programs. The overall pattern of seed proteins and amino acid composition within the same species revealed that there is a considerable variation along with the geographical region. The percentage of polymorphism for all the studied taxa was 26% and a total of 18 bands are detected within the studied Lupinus taxa, which are characterized by 4 common bands at 38, 36, 20 and 14.4 KDa. The dendrogram constructed from different concentrations of amino acid in the studied Lupinus species, demonstrated that the four studied taxa are not fully discriminated even at both different similarity coefficients and different types of sorting. By using seed protein electrophoresis, the L. albus var. albus (L. albus cultivar) is assembled with the wild L. albus below the same group. But at the same time the cultivar showed considerable degree of variability from the wild species and separated alone by using both data from seed proteins and amino acid compositions. The cultivar is characterized with both low degree of polymorphism and deficiency in arginine, leucine and methionine which are considered from the vital important amino acids. Contrary, both L. albus and L. angustifolius have considerable amounts of these amino acids.