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18505

Activation tagging in aspen using a heat-shock inducible two component Ac/Ds-enhancer element system

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Last updated: 03 Jan 2025

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Abstract

From our earlier work on the genetic transfer of the maize transposable element Ac and its functional analysis in hybrid and pure aspen lines, it was shown that this element is active in transgenic aspen. However, a two element transposon tagging system where the Ds element and the transposase gene are put together in crosses is not feasible in trees due to the in part long vegetative phases. To overcome this barrier, an inducible two element HSP-transposase /ATDs element system is suggested to induce activation tagged variants following two independent transformation steps. The ATDs element is comprised by a 35S enhancer tetramer and outwards facing two CaMV 35S promoter located near both ends of the ATDs element. Following transposition of the ATDs element expression of genes can be elevated which are located adjacent to the new integration site of the element.

Keywords

Activation tagging, rolC gene, HSP-transposase, ATDs element

Authors

First Name

Hamdy

Last Name

Atta-Alla

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-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

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First Name

Mostafa

Last Name

Zaghloul

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-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

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Orcid

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First Name

Abd El Kawee

Last Name

Waly

MiddleName

-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

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First Name

Matthias

Last Name

Fladung

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-

Affiliation

Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany

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Orcid

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First Name

Fadia

Last Name

El-Sherif

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-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

Email

woroof@yahoo.com

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Volume

4

Article Issue

3

Related Issue

3857

Issue Date

2009-11-01

Receive Date

2018-11-07

Publish Date

2009-11-01

Page Start

1

Page End

7

Print ISSN

1687-5052

Online ISSN

2090-2786

Article File

CAT_Volume 4_Issue 3_Pages 1-7.pdf

PDF . 351.0kB

Link

https://cat.journals.ekb.eg/article_18505.html

Detail API

https://cat.journals.ekb.eg/service?article_code=18505

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Original Article

Type Code

644

Publication Type

Journal

Publication Title

Catrina: The International Journal of Environmental Sciences

Publication Link

https://cat.journals.ekb.eg/

MainTitle

Activation tagging in aspen using a heat-shock inducible two component Ac/Ds-enhancer element system

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Article

Created At

22 Jan 2023