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18504

Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System

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Last updated: 03 Jan 2025

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Abstract

Based on the Ac/Ds two element transposition system from maize an activation tagging approach was suggested for the hybrid aspen (Populus tremula x tremuloides) line ‘Esch5'. A glucocorticoidinducible two element Ac/ATDs element system was used to induce activation tagged variants following two independent transformation steps. In combination with a 35S enhancer tetramer and outward facing two CaMV 35S promoter located near both ends of the ATDs element, expression of genes can be elevated located adjacent to the new integration site of the element. As selective marker for ATDs transposition, knocking-out the expression of a phenotypic marker (rolC gene) was considered.

Keywords

Activation tagging, glucocorticoid-inducible, rolC gene, Ac/Ds, transposon, ATDs element

Authors

First Name

Hamdy

Last Name

Attalla

MiddleName

-

Affiliation

Faculty of Agriculture, Suez Canal University. Ismailia

Email

hamattaeg@yahoo.com

City

Ismailia

Orcid

-

First Name

Mostafa

Last Name

Zaghloul

MiddleName

-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

Email

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City

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Orcid

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First Name

Abd El Kawee

Last Name

Waly

MiddleName

-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

Email

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City

-

Orcid

-

First Name

Matthias

Last Name

Fladung

MiddleName

-

Affiliation

Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany

Email

-

City

-

Orcid

-

First Name

Fadia

Last Name

El-Sherif

MiddleName

-

Affiliation

Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

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-

City

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Orcid

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Volume

4

Article Issue

2

Related Issue

3856

Issue Date

2018-07-01

Receive Date

2018-11-07

Publish Date

2018-07-01

Page Start

45

Page End

51

Print ISSN

1687-5052

Online ISSN

2090-2786

Article File

CAT_Volume 4_Issue 2_Pages 45-51.pdf

PDF . 2.0MB

Link

https://cat.journals.ekb.eg/article_18504.html

Detail API

https://cat.journals.ekb.eg/service?article_code=18504

Order

6

Type

Original Article

Type Code

644

Publication Type

Journal

Publication Title

Catrina: The International Journal of Environmental Sciences

Publication Link

https://cat.journals.ekb.eg/

MainTitle

Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System

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Article

Created At

22 Jan 2023