Heavy metals cannot be degraded through various treatments. Bioaccumulation and bioaugmentation of heavy metals by food chain could damage normal physiological activity of higher organisms and threat human life. Heavy metals are very toxic even at low concentration (1.0 – 10 μg/ l) and their toxicity can last for a long time in nature. Short-deep treatment beds constructed at Abo-Attwa, Experimental Station, Ismailia, Egypt were efficient in the removal of organic matter, BOD and COD from primary treated influent by 50%, 66 % and 68 %, respectively. Also, the treatment beds were efficient in the removal of 93 – 98 % of the heavy metals Cu, Pb, Zn, Ni and Cd from influent. Eight Agrobacterium strains, three A. rhizogenes and five A. tumefaciens, were isolated and characterized from naturally-occurring microbial flora proximate domestic wastewater of Abo-Attwa Experimental Station, Ismailia, Egypt. The Agrobacterium strains were able to accumulate lead, zinc and nickel at varying rates. On the other hand, copper and cadmium were toxic to all Agrobacterium strains tested. The toxicity order of various heavy metals to Agrobacterium strains tested was Cd> Cu> Zn> Ni> Pb. The three A. rhizogenes strains accumulated higher amounts of Zn and Ni than A. tumefaciens strains. Induction of some tolerant Agrobacterium strains via growth adaptation in the presence of progressive high concentration of Cd and Cu was achieved. A. rhizogenes strain A8 was superior in adaptation and survival as tolerant strain to both cadmium (Cd) and copper (Cu) on growing in Nutrient Broth containing elevated concentrations 1, 3, 10 and 30 μg/ l of both metals. The A. rhizogenes strains A1 and A7 efficiently accumulated 90 % more Cd than non-adapted Agrobacterium cells when grown at 10μg/l. Furthermore, The A. rhizogenes strains A1, A7 and A8 accumulating 90 % , 75 % and 80 % respectively more Cu than non-adapted Agrobacterium cells when grown at 10μg/l. The bioaccumulation efficiency % of the A. rhizogenes strains A1 and A7 was 40 %for accumulating 30μg/l of the toxic metal cadmium (Cd). The potential role of Agrobacterium in the bioremediation of heavy metals was also considered.