Vaccination is one of the most important strategies for fighting infection. The efficiency of vaccination is determined by many tests that evaluate the immune response vaccine elicit. Here Uropathogenic E. coli vaccines were evaluated by challenge test, indirect haemagglutination test, histopathology, haematology and biochemistry measurements. The results indicate to the benefit of inactivated polyvalent whole cell vaccine and adjuvant-use in immunopotentiation of vaccine. Many measures were used as a good indication and correlated to effectiveness of vaccine as post–vaccination antibody titer, total leukocytic count, neutrophil percent and total protein. Also the histopathology results for dead mice give clear clue to the interpretation of challenge test results.