The luminescence arising from lanthanide cations offers several advantages over organic fluorescent molecules sharp, distinctive emission bands allow for easy resolution between multiple lanthanide signals; long emission lifetimes (μs–ms) make them excellent candidates for time resolved measurements and high resistance to photo bleaching allow for long or repeated experiments. A developed method is presented for determination of nucleobase, nucleosides and nucleotides using the effect of quenching of fluorescence of the easily accessible terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex in 1:2 metals: ligand ratio. The interaction of terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex with nucleosides (NS) (cytidine, inosine), nucleobase (NB) (adenine), nucleotides (NT) (5′-GMP), (5′-IMP), (5′- ATP), (5′-CMP) and (5′-ADP) has been studied using normal luminescence techniques. The linear ranges for determination of the selected biomolecules are 1.6–36.6 μ mol.dm-3, and with limit of detection were in the range of 0.906- 8.28μ mol.dm-3.