The absorption and fluorescence measurements are performed for the interaction of Eu(III) with
synthesized coumarin derivative 3-oxo-3H-benzo[f]chromene-2-carboxylic acid (BCCA) in different
solvents. The results showed that the most suitable solvents for tracing the emission bands for Eu(III)
are methanol and DMSO. Confirmation of the formation of binary and ternary complexes Eu(III)-
(BCCA), Eu(III)-(BCCA)-(NS), Eu(III)-(BCCA)-NU nucleosides (NS)= Adenosine, Guanosine,
Inosine and Cytidine, nucleotides (NU)=5'-GMP, 5'-ATP and 5'-IMP in solution has been studied using
UV-visible spectroscopic and luminescence measurements. Also the interaction of the binary complex
Eu(III)-(BCCA) is performed with DNA using the same techniques. The binding parameters of the
binary complex with DNA and its compartments are calculated and the mode of interaction with DNA
is evaluated.