The present study provides a detailed flora composition and soil attributes of Suez Canal region. This region was represented in Ismailia-PortSaïd highway, Ismailia city and Ismailia-Suez highway which wasn't surveyed before as a whole region. The species collection was preformed seasonally from 2020 till 2022. The investigated area comprised of a wide range of habitats namely, waste land, cultivated land, irrigated areas, desert land, salt marshes, edge of cultivation and canal bank. A total of 190 species were recorded in the study area. They include 24 sub-species and 14 varieties belonging to 127 genera and 39 families. The most species-rich families were represented by Poaceae (36 species), Amarnthaceae (26 species), Asteraceae (18 species), Brassicaceae (12 species) and Fabaceae (10 species). Therophytes were the most dominate life-form in the study area represented by 58.5 percent, followed by chamaephytes (20.5%), hemicryptophytes (7.8%), cryptophytes (7.89%), then phanerophytes (4.7%) and parasites 0.5%. This study revealed seventeen species as new records to the flora of the study area. Out of these six species are added as new records to the flora of the Mediterranean region, Eastern desert and Isthmic desert.