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78378

Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

2. Cytotaxonomy

Abstract

Phenetic and phylogenetic relationships between ten Hordeum vulgare L. taxa , seven cultivars (Giza 123, Giza 126, Giza 127, Giza 128, Giza 129, Giza 130 and Giza 2000) and three landraces collected from Sinai (El-Kheroba, El-Sheikh Zuwaid and Wadi Sedr). This study was carried out using morphological criteria, molecular genetic markers (SSRs) and finally soluble protein patterns. The results of morphological traits reflected that Giza 2000 and Wadi Sedr gave superior in vegetative growth and yield productivity in contrast to Giza 129, which showed an inferior vegetative growth and yield. The SSR analyses using 7 primers and revealed detection of a total of 15 bands, among which 8 bands (61.22%) were polymorphic. Finally we can conclude that, the genetic distance tree was produced by UPGMA based on morphological data stated the pedigree of studied cultivars. However, in the tree based on molecular markers (SSRs) and soluble proteins patterns were not in full agreement with the pedigree.

DOI

10.21608/taec.2020.23618.1015

Keywords

Hordeum vulgare L, genetic relationships, morphological Criteria, SDS-PAGE Electrophoresis, Molecular markers (SSR-PCR)

Authors

First Name

Samira

Last Name

Osman

MiddleName

Ali Hassan

Affiliation

Genetics and Cytology dept., Genetic Engineering and Biotechnology dev., National Research Centre, Giza, Egypt

Email

s_nrc82@yahoo.com

City

cairo

Orcid

0000-0001-7764-5139

First Name

Essam

Last Name

Mostafa

MiddleName

Ahmed

Affiliation

Genetics and Cytology dept., National Research Centre, Giza, Egypt

Email

dressamahmad@gmail.com

City

Giza

Orcid

-

First Name

Zeinab

Last Name

El-Ashry

MiddleName

Mohamed

Affiliation

Genetics and Cytology dept., National Research Centre, Giza, Egypt.

Email

aaah-a@yahoo.com

City

-

Orcid

-

First Name

Fawzia

Last Name

Mohamed

MiddleName

Ismail

Affiliation

Genetics and Cytology dept., National Research Centre, Giza, Egypt.

Email

adam_fwz@yahoo.com

City

Cairo

Orcid

-

First Name

Hala

Last Name

Elatroush

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Ain Shams University, Cairo

Email

hala_elatroush@hotmail.com

City

-

Orcid

-

First Name

Soheir

Last Name

El-Khodary

MiddleName

-

Affiliation

Botany dept., faculty of Science, Ain Shams University.

Email

sssss_dd@yahoooo.com

City

-

Orcid

-

Volume

40

Article Issue

1

Related Issue

10428

Issue Date

2020-01-01

Receive Date

2020-02-07

Publish Date

2020-01-22

Page Start

27

Page End

40

Print ISSN

1110-7413

Online ISSN

2357-044X

Article File

TAEC_Volume 40_Issue 1_Pages 27-40.pdf

PDF . 928.0kB

Link

https://taec.journals.ekb.eg/article_78378.html

Detail API

https://taec.journals.ekb.eg/service?article_code=78378

Order

3

Type

Original Article

Type Code

636

Publication Type

Journal

Publication Title

Taeckholmia

Publication Link

https://taec.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023