Background: COVID-19 pandemic resulted in a global social isolation and personal distress, which may affect sexual function of both women and men. Aim: This study aimed to assess the effect of COVID-19 pandemic on the sexual function of women and men. Design: A descriptive cross-sectional research design was utilized. Setting: The research was conducted at two health centers located in Senbellawein City, Dakahlia Governorate, Egypt. Subjects: A non-probability purposive sample of 202 participants (105 women and 97 men) was included. Tools: Three tools were used: A structured interview questionnaire, Arabic versions of the Female Sexual Function Index and the International Index of Erectile Function questionnaires. Results: The current study findings revealed that there was a significant increase in sexually avoidant behaviors and taking precautions during intercourse such as using condoms and avoidance of kissing during the COVID-19 pandemic. Also, there was a highly significant reduction in sexual intercourse frequency of both women and men. In addition, a highly significant decline in arousal, lubrication, orgasm, and satisfaction domains were observed among women. Furthermore, a significant decline in erectile and orgasmic functions, intercourse satisfaction, and overall satisfaction scores among men. Conclusion: The sexual function of both women and men was affected, probably, due to the worldwide stressors of the emergent COVID-19 pandemic. Recommendation: Psychological support should be provided to all couples to enhance coping with COVID-19 related stressors. Counseling couples that the active sexual relation should be maintained during the pandemic while following the recommended protective measures.