Background: Nurses are the largest and essential capital of health care organizations. Nurses' intention to leave is a devastating phenomenon that threatens the health care systems locally and globally. Work practice environment and workplace civility climate are major determinants of nurses' intention to leave. Aim: To evaluate the relation between work practice environment, workplace civility climate and staff nurses' intention to leave the profession. Research Design: A descriptive correlational research design was utilized. Setting: Different departments at Beni-Suef University hospital. Sample: A convenient sample of 165 staff nurses were recruited for the study. Data Collection Tools: Three data collection tools were used: Practice Environment Scale of the Nursing Work Index (PES-NWI), Perceived Workplace Civility Climate Scale (PWCCS) and Staff Nurses' Intention to Leave Nursing Profession Questionnaire. Results: More than three quarters of staff nurses (80%) perceived their work practice environment as unfavorable. More than half of the staff nurses (56.3%) had moderate level of perception of work place civility climate. About three quarters of staff nurses (74.5%) had high intention to leave.  There was a significant negative statistical correlation between work practice environment and staff nurses' intention to leave (P=0.000). Also staff nurses' intention to leave was negatively correlated with perceived workplace civility climate (P=0.000). Work practice environment was positively correlated with work place civility climate (P=0.002).  Conclusion: The study concluded a highly statistically significant relation between work practice environment, workplace civility climate and staff nurses' intention to leave the profession. Recommendations: Adopting new leadership approaches and practices that are supportive to staff and building a constructive work environment and improving workplace civility climate. Make the necessary changes in the work practice environment based on the suggestions and recommendations of staff nurses.