Background: Professionalism in nursing reflects the act of providing quality patient care while honoring the values of respect, advocacy, and responsibility. Professionalism also extends to a nurse's ability to communicate clearly and self-reflect on behaviors and actions, always striving for both professional and personal development Research Aim: to assess the effect of a training program on improving intern nursing students' professionalism Research design: A quasi experimental design was utilized from January 2021 - May 2021. Research Setting: The study was carried out at faculty of nursing affiliated to Fayoum University Sample: The subjects were 165 intern nursing students through convenience sample. Tool: A self-administered questionnaire after translated to Arabic language, containing three parts (Demographic characteristics, Professionalism scale) Results: Mean score of scientific and technical domains pre intervention were 6.87 and , while post intervention was  and 10.90±3.24 and follow-up were 11.80±3.24 and 10.13±2.80. Additionally, mean score of face to face interview and peer review domains pre intervention was 5.60±1.34 and 7.12±2.56, while post intervention were 10.50±2.24 and 13.21±3.01 and follow-up were 10.03±2.65 and 12.43±2.70. Conclusions: This study concluded that more than two thirds of studied intern nursing students had low professionalism pre intervention, while improved to more than half of them had high professionalism post intervention and follow-up with high significant difference at p value <0.01**. Recommendation: Provide education program for head nurses about benefits of achieving professionalism at work. Assess the factors that hinder achieving professionalism at nursing.