Background: Documentation is reported to take up to 50% of nurses' time per shift Training, either on-the-job or off-the-job, is the only means that may improve such lack of competencies in documentation. Aim of the study: To investigate the impact of documentation practice training program for nurses: on-the-job vs off-the-job. Design: a quasi-experimental design was used. The study was conducted at Damanhour National Medical Institute. Subjects: 210 nurses took part in this study. The questionnaire was used for data collection; the first part related to demographic characteristics, the second part: This part to assess the nurse's knowledge gained from the training. The third part included the Audit sheet, which was intended to assess the nurse's practice of documentation. The intervention consisted of a 2-day training in nursing documentation. Results: high statistical significances differences between studied nurses at pre, post and follow up intervention related to in the knowledge and audit scores of the nurses in the on-the-job vs off-the-job. The Mean ±SD knowledge score increased from 38.9± 9.81to 78.32, and the Mean ±SD total audit scores from 15.3± 4.8 to 39.98±8.57. Likewise, in the off-the-job group Mean ±SD knowledge score rose from 39.67± 0.21to 75.91±7.10, and the Mean ±SD total audit scores from 14.94±8.12 to 38.52±7.97. Conclusion; highly statistically significant improvement in total staff nurses' documentation knowledge and audit level during post and follow up phase as compared with preprogram phase. Recommendations: Continuous training programs or sessions must emphasize on all aspects of nursing documentation.