Background: COVID-19 epidemic is rapidly increasing throughout the world . It is an important public health crisis which threat the humanity. Pediatric nurses are at the frontline of fighting against COVID-19. Nurses are faced with various challenges through the provision of care to provide high quality of nursing care for children and their family. So, this study aimed to identify the challenges facing pediatric nurses throughout the provision of nursing care for children with COVID-19. Design: Descriptive research design was used. Setting: This study was carried out at isolation pediatric unit in Assiut University Children Hospital. Subjects: Convenience sample of (33) pediatric nurses have participated in this study. Two tools were used in this study. Tool I: Structured interview questionnaire sheet for nurses. Tool II: Challenges facing pediatric nurses during COVID-19 crisis. Results. High Mean and SD of psychological challenges of pediatric nurses followed by challenges related to the organization's inefficiency in supporting nurses, physical challenges and finally challenges related to the nature of the disease (covid19) with Mean and SD were 12.9 ± 2.66 ,11.48±2.43, 11.36±1.78 & 8.0±1.80 respectively. Conclusion: Most of the studied pediatric nurses faced high challenges during COVID 19 and there was a statistically significant relationship between the total challenges level and personal data of the studied nurses except residence. Recommendations: Training programs are necessary to empower nurses with applicable preparations & manipulation for the challenges and experiences facing them to improve the skills that enable them to deal with challenges that help in controlling of crisis.