Background: Coronavirus infection (COVID-19) is a pandemic illness that is especially common in health-care setting, nurses' educational institution must take efforts to protect themselves and others throughout the outbreak by preventing, isolating, and caring for infected Covid-19 patients. As a result, a high-quality nursing education program positive improve the quality of nursing care. Aim: To investigate effect of nursing educational program on Nurses′ knowledge and practices regarding pandemic Covid-19 in isolation Unit and chest department. Study design: Quasi-experimental research design was utilized in this study.   Setting: The study was conducted in isolation unit and chest department at Assiut University Hospital. Sample: A convenience sample of all available nurses working in isolation unit and chest department (30 nurses). Tools: Two tools were used for data collection, Tool (I): Critical care nurses' knowledge assessment questionnaire sheet, Tool (II). Critical care nurse's practices observation checklist tool. Results: the two stated research hypotheses can be supported with (p value<0.001** ), nurses' total knowledge and total practices respectively should an improvement post educational program implementation and a positive correlation were found between them with (r =0.370  p value =0.044**). Conclusion: Application of educational program had statistically significant effect on knowledge and practices of nurses regarding caring of covid-19 patient. Recommendation: In-service training program for nurses are needed to keep their knowledge and practices up to date on the management of covid-19 patients.