Background: Central Line Associated Blood Stream Infections (CLABSIs) are the most
common healthcare associated infections in the Neonatal Intensive Care Unit (NICU). The nurse has an
important role in CLABSI bundle during insertion and maintenance. This study aimed to: Assess
nursing knowledge and compliance regarding CLABSI bundle. Setting: This study was conducted at
the (NICU) in Children University Hospital (Abu Elrish) El-Mounira and New Kasr El-Ani Teaching
Hospital. Design: A descriptive design was used. Sample: A purposive sample of 86 nurses included in
the study working at the previously mentioned setting. Tools: The first tool is a predesigned
questionnaire to assess nurses' knowledge about CLABSI bundle; the second tool was observational
checklist to assess nurses' compliance for neonates with CVL. Results: Revealed that less than half
had poor knowledge and more tham half had average knowledge about CLABSI bundle while more
than half had incompetent practice. Conclusion: The current study provides a picture of the current
nursing knowledge and compliance of CLABSI bundle in NICUs. The study conducted that less than
half had poor knowledge and less than half had average knowledge about CLABSI bundle while more
than half had incompetent practice. In addition, the study illustrated an absence of a uniform protocol
for prevention of CLABSI in the studied NICUs and absence of adequate training. This indicates the
need for developing a protocol for CLABSI prevention based upon current evidence based guidelines
and make education program on this protocol. There is also a need for establishing a system to
implement prevention protocols and the monitoring of adherence and CLABSI bundle.
Recommendations: Collaboration and continuing education of the staff in the NICUs are vital to
improve their knowledge and practices of care provided for neonates with central venous line.
Standardized nursing guide lines about CLABSI bundle should be used to guide the nurse while caring
of neonate with central line. Make checklist for insertion and maintenance CLABSI bundle for each
patient and also make kits for CVL insertion and dressing change.