Background: COVID-19 seriousness may result from its pandemic infection from human-to-human transmission and evidence of transmission from animal to human as well. Mainly COVID-19 is transmitted by respiratory droplets while aerosol transmission can occur in specific situation mainly in crowded poor ventilated spaces. This transmission affects individual, health care workers (HCWs), health institutions and the whole community Aim of the Study: to identify factors associated with transmission of COVID-19 infection among nursing staff in Fever hospital, Kafr Eldawar, 2021. Research Design: Cross sectional research design was used. Setting: The present study was conducted at This study was conducted in Kafr Eldawar Fever hospital. Subjects: A convenient sample of 104 nurses out of total number of hospital nursing staff (who are on duty, accessible at the time of the study and agreed to participate in the study) is 169 nurses in all hospital units. Tools: one tool was developed by the researchers in the form of self-administered questionnaires “factors associated with COVID-19 infection". It consisted of four parts, part I: socio-occupational data, part II:" Factors predisposing nursing staff to COVID-19", part III: "Adherence to infection prevention and control (IPC) measures information" and part IV: " Nursing staff opinions". Results: Majority of nurses were directly assigned beds to patients diagnosed with COVID-19, more than two thirds of them worked in a COVID-19 isolation ward and were touching their cheeks, nose, or mouth at work. Conclusion: There is significant relation between Participation in in-service training program related to dealing with COVID-19 infection and previous COVID-19 infection and factors predisposing one to COVID-19. Recommendation: Well-established hospital information systems (MIS), personal digital assistant (PDA) systems, and a local intranet to avoid unnecessary contact with COVID-19 infection.