Background: An important factor influencing duration of breastfeeding is mother's employment status. Aim: To investigate the effect of educational program on pregnant knowledge and practice regarding how to continue breast feeding after returning to work. Design: A quasi-experimental design was utilized. Setting: The study was conducted at Outpatient Clinic and High risk Units of special Hospital at Beni- Suef University. Sample: A convenience sample of all available pregnant working women (300 women) from the previously mentioned setting. The following study tools were used (pre/posttests): 1) Structured interview questionnaire to assess pregnant working women` s knowledge regarding how to continue breast milk feeding after returning to work. 2) Working women`s practice observation checklists to assess pregnant working women`s practicesuch as hand washing, breast care and nipple cleaning, breast massage, Daily shower, Finding the perfect pump, Staying pumped and Maintaining supply Boosting milk supply and Storing and reusing milk.Results: The mothers' age ranged between 30< 34 years with mean age 31.03 ± 4.50and 45%of them had Secondary/higher educations. There was a highly statistically significant difference (P<.0001), regarding their knowledge and practices before, immediately after, and at follow up guideline implementation about how to continue breast milk feeding after returning to work.Conclusion: The present study concluded that, the educational guideline had a positive effect on mothers' knowledge and practice about how to continue breast milk feeding after returning to work. Recommendations: Provide continuous education and training for working women regarding how to continue breast milk feeding after returning to work. Supporting legislation to provide private, hygienic lactation rooms to ensure the safe storage of expressed milk.