Background: Brucellosis is an occupational hazard for slaughter house workers. Community health
nurses have an important role in training them in safe practices for prevention. Aim of the study: to
evaluate the effect of health guidelines for slaughter house workers about brucellosis in Port Said.
Subjects and methods: The study was conducted on all 70 workers employed at Port Said slaughter
house. It is located in El Gabbouti Al-Gadeed at AL-Ganoob district by using a one-group quasi
experimental research design with pre-post assessment. Data were collected using a structured
interview questionnaire form for worker's knowledge of brucellosis, observation checklists for safe
practices and hand washing, and a laboratory sheet for the results of brucellosis blood tests. Based
on the information obtained from the initial assessment, the researchers designed the health
guidelines and implemented it. Results: All workers were males with median age 42 years. Overall,
83.1% of the tested samples (n=65) demonstrated sero-positivity. Only 2 (2.9%) workers had
satisfactory knowledge before the intervention, which increased to 70.0% after the intervention
(p < 0.001). Moreover, 32.9% had adequate practice before the intervention, which rose to 92.9%
after the intervention (p < 0.001), with significant improvement in hand washing practice (p < 0.001).
In multivariate analysis, the study intervention was the main significant independent positive
predictor of the scores of knowledge, hand washing practice, and total practice; the knowledge score
was a significant positive predictor of the score of hand washing practice. Conclusion and
recommendations: The implementation of health guidelines can be effective in remedying the
deficiencies in slaughterhouse workers' knowledge and practices. Therefore, the guidelines should
be implemented in the setting and similar ones