Background: Mechanical ventilation (MV) is a common procedure and life-saving used to treat patients with respiratory failure. Aim of the study: This study aims to evaluate the effect of self-learning package on nurses' performance caring for patients on ventilators. Subjects and Methods: Research Design: A quasi-experimental study was conducted in critical care units: ICU; RICU and CTICU at Ain-Shams University Hospitals. Subjects: A convenient subject of all available nurses caring for patients on MV were recruited for the conduction of this study from the above mentioned setting. Tools: (1) Self administered questionnaire: Part one is concerning with demographic data that include (age, gender, educational levels, work area, years of experience, ect..). and Part two is concerning with nurses' knowledge regarding caring for patients on MV, (2) Nurses' performance observational checklists: It used to assess nurses' practices during caring for patients on MV and(3) Nurses' opinions questionnaire regardingself-learning package (SLP) and problems facing nurses during management of the patient on MV: It was used to assess nurses' opinions regarding using SLP and problems & barriers facing nurses. The results of this study reveal that, the mean age for nurses under study was 31.38 ± 6.88. More than two thirds of nurses were females and more than half of them were Diploma nursing graduates. Also, there was statistically significant improvement immediately after SLP implementation and throughout the follow-up phases regarding nurses' performance related to care of patients on ventilators. Conclusion: Concluded that there was statistically significant positive effect for SLP on nurses' level of knowledge and practice. Recommended: this study recommended conducting the SLP in a wider field including all hospitals to raise the efficiency of nursing care provided.