The present study aimed to investigate nurses' compliance to safety measures at labor
room. Research hypothesis: Utilization of instructional guideline for safety measures will
enhance a positive impact on nurse's knowledge and practices at labor room. Subjects and
methods: A quasi experimental study design was used and conducted at labor room in Ain
shams Maternity University Hospital. Convenient sample was used to recruit 45 nurses with
different ages, qualifications and experiences) during the morning shift. Three tools were used for
data collection. First tool A Structured interview questionnaire sheet. Part (1), assessed the
demographic characteristics of study sample, part (2,) assessment of knowledge regarding safety
measures compliance and nursing care for labor and part (3) barriers in application of safety
measures at labor room. Second tool: An observational checklist to assess nurses practices both
safety measures compliance and nursing care in labor. Third tools: Likert scale to assess nurses'
satisfaction regarding utilization of the instructional guidelines. Results: revealed highly
significant differences between the pre and post knowledge level and nursing practices also there
were significant correlation between socio demographics characteristic with their both nurse
knowledge and practices pre and post intervention. Conclusion: It was concluded that, there were
relation between nurse's knowledge and their practices regarding to both safety measures and
nursing care at labor room (post intervention), there was positive impact on nurse's knowledge,
practices and compliance at labor room. Recommendation: it was recommended that, there was
needed training program to overcome barriers as nurse's performance and compliance regarding
safety measures in all maternity care units.