Background: Reproductive tract infections (RTIs) are recognized as a major public health problem and rank second - after maternal morbidity and mortality - as the cause of healthy life loss among sexually- active women of reproductive age in developing countries. The aim: the study: The study aimed to assess to assess knowledge &practice of women regarding lower of reproductive tract infection. Setting: The study was conducted at the patient gynecological clinic at Benha University Hospital. Design: Descriptive study design. Sampling: A purposive sample included300 women were recruited in the current study. Tools: Three tools were used to collect data, Tool 1-Interviewing questionnaire to collect data include Part 1:-sociodemographic characteristics data, Part 2:- Reproductive history Part 3:- Related to characteristics of vaginal discharge Part 4:- Related to personal hygiene habits Tool II: - women's knowledge and Tool III-An observation check list: used to assess practice of studied sample of reproductive tract infections. Results: The present study revealed that most common of the studied group are mean age 25.67 ± 2.09 married, house wife, and are living in urban area. There is highly statistically significant differences women's total knowledge score and their marital status, age at marriage, and years of marriage. The total practice score in relation to their personnel characteristics, it reveals that there was a statistical significant difference between women total practice score and their residence, age at marriage, and years of marriage. Conclusion: There is highly statistically significant differences women's total knowledge score. And the total practice score in relation to their personnel characteristics, it reveals that there was a statistical significant difference between women total practice score and their residence, age at marriage, and years of marriage in addition to highly positive significant correlation that clarifies that increase knowledge will subsequently increase practice of women regarding lower reproductive tract infection. Recommendations: Periodic educational program for women to increase their knowledge and to improve their practice. Counseling women about the importance of periodic follow up.