The aim of the current study is to evaluate the effect of guidelines on early detection of cervical cancer among high risk group women. A Quasi experimental design was used. The study was conducted in Early Detection Unit at Ain Shams University Maternity Hospital. a purposive sample for One hundred women were included in the study. Three tools of data collection was used named cervical cancer interview Arabic questionnaire sheet, follow up sheet, diary sheet. The main results of the study indicate that highly statistical significant difference between women knowledge regarding cervical cancer before and after using guideline. As regard to, total attitude score regarding cervical cancer before & after using guideline the present study revealed that highly statistical significant difference between women attitude regarding cervical cancer before and after using guideline. The current study concluded that, the nursing guideline influence on women screening follow up for cervical cancer among high risk group women., Nursing guideline enhance women knowledge & attitude positively regarding cervical cancer. Based on this finding, the researchers recommended; there is an urgent need for conduction of educational programs targeting women by trained health care providers to providing information regarding cervical cancer.