This study aimed to assess knowledge and satisfaction among mothers with gestational diabetes (GDM). A descriptive study design was utilized. The study was conducted at outpatient clinics and inpatient departments of Ain Shams Maternity University Hospital, on a convenience sample consisting of 200 gestational diabetes mothers. Two tools of data collection were used: a structured interview questionnaire, and ―satisfaction part‖ of QOL scale to evaluated satisfaction among gestational diabetes mothers. The study results showed that, half of studied mothers were satisfied with a correct knowledge regarding gestational diabetes management as well as 85.3% of them were satisfied with management of hypoglycemia. There were highly significant association between mothers' knowledge and their age, education, and area of residence. Also the results revealed that, studied mothers were satisfied with social/economic, psychological/spiritual and family domains (66%, 64% & 50.5% respectively); however 65.5% of them were un-satisfied with health/functioning domain. The study concluded that, the studied mothers had correct knowledge regarding management of GD, effect of GD on mother's health, effect of GD on fetus health, management of hypoglycemia, and measuring blood glucose at home (62.1%, 64.2%, 67.4%, 90.5% & 63.2 & respectively). More than two thirds of the studied mothers were more satisfied with social/economic and psychological/ spiritual domains and GDM affects studied mother's health and functioning domains Recommendation: Developing awareness program to enhance pregnant mothers' knowledge and satisfaction regarding promotion of their health in relation to gestational diabetes