Background: Pediatric nurses had unsatisfactory knowledge and practice regarding the care of nephrotic syndrome children as reported by many studies. Aim: This study aims to evaluate the effect of an-educational program on knowledge and practice of nurses who are caring for nephrotic children. Methods: A quasi- experimental design was utilized in current study. Sample: Convenience sample of 57 pediatric nurses who are caring children with nephrotic syndrome. Tools of data collection: Two tools were utilized for data collection; self- administered questionnaire sheet included personal characteristics of the pediatric nurses, their knowledge, and observational checklist for evaluation of nursing practices of children with nephrotic syndrome. The results Shows that majority of the pediatric nurses had unsatisfactory knowledge and inadequate practice about nephrotic syndrome before program implementation, that improved significantly immediate and 3 months after program implementation, there are a positive correlation between nurses' knowledge, age and years of experience, also between nurses practice, age, years of pediatric and general experience. Conclusion: The pediatric nurses' knowledge and practice improved significantly after program implementation and this improvement positively correlated with age and years of experience. The study recommended that in service training program should be conducted for the pediatric nurses in their work place to update their knowledge and improved their practice.