Hemophilia is a coagulation disorder arising from a genetic defect of the X chromosome; the defect can either be inherited or result from spontaneous gene mutation. In each type of hemophilia (A, B, C, and willebrands disease), a critical coagulation protein is missing, causing children to bleed for long periods of time before clotting occurs. Aim: this study was a quasi- experimental aimed to evaluate the outcomes of the instructional module on the studied sample. Setting: the study was carried out at hematology departments at children's hospitals affiliated to Ain Shams University Hospital (Al Demerdash) Cairo University Hospital (Abo Elreesh) and Benha children's Hospital affiliated to ministry of health. The Sampling: A convenient sample involved Fifty nurses from the previously mentioned settings. Tools of data collection: involved a structure questionnaire to assess nurses' knowledge and observational checklists to assess performance regarding care of children with hemophilia and instructional module about hemophilia. Results: Slightly less than half (46.0% &48%) of the studied nurses their ages were 30 years and more, had nursing diploma from technical institute of nursing respectively, less than two thirds of the nurses (62%) had experience ≥ 6years, Post implementation of the instructional module most of the studied nurses had satisfactory level of knowledge and competent practice in comparison to pre implementation of the instructional module. Conclusion: The designed instructional module led to significant improvement of knowledge and performance of the studied nurses towards care of hemophilic children. Recommendations: continuous refreshing training should be provided for all of nurses working in hematological departments and caring of children with hemophilia. A manual guided booklet about hemophilia should be available to all nurses working in hematological departments.