Background: Sexually transmitted diseases are major global public health problems that are the main cause of acute illness, infertility, long-term disability and death. Aim of this study was to evaluate the effect of educational guidelines nursing program regarding sexually transmitted diseases among male nursing university students. Quasi-experimental pre-post -test design was utilized to fulfill the aim of this study. A Purposive sample of a total 108 male nursing students which included 75 in 1st academic year & 33 in 2nd year at Faculty of Nursing Benha University. Data were collected through a structured interviewing questionnaire. Results: there was a highly statistically significant difference related to all items of knowledge regarding STDs pre -test and immediately after program implementation (P< 0.001**). Conclusion: the current study found that a two session educational intervention was beneficial in increasing STDs knowledge of university students. Recommendation: Application of continuous comprehensive health education programs is recommended for students in different grades to focus on STDs with implementation of a practical training course to improve the nursing students' knowledge.