Aim: Aim of this study is to determine the effect of nursing protocol on the third and fourth stages of labor. Setting: The study was conducted at obstetric and gynecology department in benha university hospital. Design: A quasi experimental design was utilized. Sampling: A purposive sample included (80) nurse. Tools: The tools of data collection were interviewing questionnaire sheet and observational checklist. Results:. More than two thirds of the studied nurses had poor knowledge regarding third and fourth stage of labor was poor in pre intervention test and improved to more than three quarter in post intervention test P value<0.001**..Majority of nurses had unsatisfactory practices in pre test as compared with post intervention test P value <0.001** .Conclusion: More than two thirds of the studied nurses had poor score of knowledge in pre test and improved to more than three quarter had good knowledge in post test. Majority of studied nurses had unsatisfactory practices and in pre intervention test as compared with post intervention test and majority of them had satisfactory practice. Recommendation: Educational program for nurses to upgrade their knowledge and practices regarding the third and fourth stages of labor.