Background and study aim: Gastrointestinal (GI) endoscopy is an important method for diagnosis and treatment. Endoscopes are complex and reusable devices. Understanding infection control in the context of endoscopy is important in reducing the transmission of infection. The infection control intervention program considers the most effective and important for improving nurses' knowledge and practice regarding infection control measures for patients with esophageal varices during endoscopy. This study aim  to evaluate effect of nurses intervention regarding infection control measures for patients with esophageal varices during endoscopy at Zagazig University Hospitals. Subjects and Methods: A quasi experimental design was used in this study. The study was conducted in endoscopy units at Zagazig University Hospitals. The study sample all available endoscopy nursing staff. The study lasted from the beginning of Jun 2017 to the end March of 2018. Results: Revealed that, slightly more than half of nurse's were in the age group of more than 40 years with mean age Mean±SD 38.7 ± 10.7 years and had more than 10 years of experience. There was improvement in total level of nurses' knowledge 0.0% pre nursing intervention increased to 100% post nurses intervention and practice regarding infection control Mean±SD 9.8±3.7 pre nursing intervention increased to Mean±SD 96.2±0.7 post nurses intervention with highly statistically significant difference between pre and post program regarding to infection control. Conclusion: It can be concluded that the implantation of health intervention program had improved nurse's knowledge and practice regarding endoscopy infection control. Continuous intervention infection control training programs are recommended in endoscopy units.