Background and study aim: Helicobacter pylori infection is distributed worldwide. H. pylori colonize the liver and increase the severity of the liver pathogenesis. The aim of the present study was to detect the H. pylori in the HCV patients using serology and immunohistochemistry diagnostic methods. The aim was extended to evaluate the hematological and biochemical changes during the co-infection. Materials and Methods: ELISA and immunohistochemistry diagnostic methods were utilized to examine patients chronically infected with HCV for H. pylori, and some hematological and biochemical parameters were detected. Results: Overall prevalence of H. pylori infection was 10.81%, 8.00%, using ELISA, and immunohistochemistry, respectively. In the current study, no significant difference based on gender, residence, age, biochemical assessment and HCV RNA load was observed. Liver cirrhosis at patient co-infected with H. pylori and HCV recorded high percentage compared to those with chronic HCV mono-infection. A significant increase in the relative lymphocyte count was detected in patients with concomitant H. pylori and chronic HCV infections compared to patients with chronic HCV mono-infection. Conclusion: Because H. pylori infection is frequent among Egyptian HCV infected patients, regular screening and treatment for H. pylori among this category is extremely important.