Background: Catheter-associated urinary tract infection is the most common hospital-acquired infection which is treated empirically by quinolone antibiotics. Analysis of clinical characteristics, risk factors, and common pathogens has a major effect on the advancement of the prevention of CAUTI in medical settings. Subjects and Methods: Urine samples were collected from Intensive Care Units at Mansoura University Main Hospital over two years. Samples were then transferred to the Microbiology lab, at Mansoura University Main Hospital for culture, identification, and sensitivity Results: The most common pathogens were E. coli (27.4%) followed by Candida (21.2%) and Staph aureus (12.0 %). Females were slightly higher (52 %) than males (48 %). diabetes appeared to be the most common risk factor (31% of cases) followed by chronic kidney disease (23.5%) and cerebrovascular accidents (23%). Meropenem was the highest sensitive antibiotic (48.9%) followed by amikacin (47.4%), norfloxacin (46.2%), imipenem (41.8) while cefuroxime (100.0%), and cefaclor (100.0%) are the most resistant antibiotic followed by ceftriaxone (97.0%), doxycycline (96.0%). Conclusion: cases in our study showed a high resistance rate to quinolone antibiotics which need infection control plans and antibiotic stewardship programs to reduce that rate. Keywords: CAUTI, Quinolones, E coli.