Background: Over the past several decades, we have witnessed a significant shift in healthcare delivery from the acute, inpatient hospital setting to a variety of outpatient settings. Much of the inpatient care is now delivered in outpatient settings, using invasive procedures and advanced technologies, which increase the risk for HCAIs. Objective: To evaluate the role of educational program in eliminating infection potential hazards inside gynecology and obstetrics clinic. Material and Methods: Three phases interventional study included before education (phase I) for 3 months, after education (phase III) for 3 months, and interventional phase of 1-month (phase II) in which educational sessions about IC standard precautions, environmental cleaning and reprocessing medical devices done. Result: Contamination level in phase I was 77.8% in bed, 83.3% in table, 63.9% in stethoscope, 80.6% in U/S abdominal probe, 50% in vaginal speculum after cleaning, and 16.7% in vaginal speculum after sterilization. This level decreased in phase III to 38.9% in bed, 38.9% in table, 30.6% in stethoscope, 27.8% in U/S abdominal probe, 13.9% in vaginal speculum after cleaning, and 0% in vaginal speculum after sterilization. The indicator organisms isolated were [MRSA, Pseudomonas spp., Acinetobacter spp. E. coli, and Klebsiella spp.]. (100%) S. aureus isolates (48/48) were MRSA, (100%) Acinetobacter spp. (15/15), E. coli (5/5), and Klebsiella spp. (3/3) were multidrug resistant (MDR), and 88.2% (15/17) of Pseudomonas spp. isolates were MDR. Conclusion: The educational program in phase II succeeded in achieving a statistically significant reduction in contamination level (p≤0.05 at all sites), also achieved a decrease in number of indicator organisms found in all sample sites.