Background: Combined micro-needling with platelet-rich plasma (PRP) is an effective treatment option for atrophic post-acne scars. Lyophilized PRP (L-PRP) is a novel, simpler, standardized preparation of PRP. The combined treatment of post-acne scars with micro-needling and L-PRP hasn't been previously investigated. Objectives: This study aimed to compare the effectiveness of the combined use of micro-needling and L-PRP versus micro-needling only in treating atrophic post-acne scars. Patients and methods: This randomized controlled split-face study included 20 adult patients with post-acne atrophic scars.  One side of the face was treated with micro-needling. The other side was treated with micro-needling and L-PRP. The treatment was applied at 2 weeks intervals for 3 sessions. The assessment was done with Antera 3D^® camera analyzer. The mean indentation index of each side of the face was calculated before and two weeks after the last treatment. Results: Our study showed that the mean age of patients was 28.35 ± 4.87 years, 90% were females, and 20% were males. 50% had Fitzpatrick skin type III, while the other half had skin type IV. Both micro-needling monotherapy and combined micro-needling with L-PRP produced significant improvement in the mean indentation index of atrophic post-acne scars. However, the mean percentage of improvement was higher in the combined treatment group. Side effects were minimal and transient with both treatment modalities. Conclusion: Combined treatment with micro-needling and L-PRP showed superior results in improving atrophic post-acne scars and is regarded as a safe, simple, and cost-effective treatment modality.