Background:Diabetic dyslipidemia is an important factor in the development of diabetic vascular complications. It may be caused or aggravated by variants of the genes coding for enzymes and proteins involved in lipoprotein metabolism as lipoprotein lipase (LPL).  Objective: To identify the genotype distributions of LPL(rs320) and (rs1801177) and to study its associative role in the development of diabetic dyslipidemia in Egyptian patients. Subjects & Methods: This cross-sectional case-control study was conducted on 200 subjects, 140 patients with T2DM and 60 sex- and age-matched control subjects. Lipoprotein lipase (LPL) gene variants (rs320 & rs1801177) were genotyped by real-time PCR using the allele discrimination by TaqMan assay. Results: Diabetic patients with dyslipidemia had a higher frequency of TT (wild) genotype of LPL (rs320) in comparison to non dyslipidimic patients (p=0.034). However, there was no difference between any of studied groups regarding LPL (rs1801177) genotype distributions. Mutant variant of LPL rs1801177 gene of diabetic dyslipidemia group was associated with increased triglycerides (TGs) , cholesterol, low density lipoprotein cholesterol (LDLc) (p= 0.001), & decreased high density lipoprotein cholesterol (HDLc)  (p=0.004) .Also, mutant variant of LPL rs320 gene had higher levels of TGs, cholesterol, &  LDLc levels (p= 0.011, 0.001, 0.001 respectively), and lower levels of HDLc cholesterol (p=0.001) in the same group. Conclusions: Association was detected betweenLPL(rs320) & (rs1801177) gene variants and dyslipidemia in diabetic patients. The interaction of LPL (rs320) and LPL (rs1801177) possibly plays a role in diabetic dyslipidemia.