Introduction: Our plant under research, Cymbopogon citratus, commonly referred to as lemon grass and a member of the family fabaceae, is one of those potential medicinal plants. It is known as a medicinal plant and is significant in various uses, particularly pharmaceutical usage due to the presence of the active compounds represented by terpens, phenols, alkaloids, etc. Our plant under study is one of these significant medicinal plants. Objective: In this work, lemongrass was harvested from the desert of the Anbar Region and then extracted using a Clevenger device to produce oil. To identify the phytoconstituents found in the Cymbopogon citratus plant, this study was done. Materials and Methods: The sample was identified by GC-Mass, which indicated the presence of 2-Methyl-Z, 13-octadecadienol with percentage 39.86%. Alkaloids, phenols, tannins, and flavonoids were also present, however cardiac glycosides and saponins were not. Results: We isolated certain pure phytopharmaceuticals, which may then be employed as a lead molecule to create a new drug with potent therapeutic effects. The antibacterial activity of lemon grass oil on microorganisms has been investigated utilising the well diffusion method to battle pathogenic bacteria and zone of inhibition of growth with widths of 22 mm. Conclusion: Using the well diffusion method, the antibacterial activity of lemongrass oil on microorganisms has been investigated in order to battle pathogenic bacteria and zones of inhibition of growth with diameters of 22 mm.