Background: Despite the safety and efficacy of mRNA COVID-19 vaccines against SARS-CoV-2, immunisation rates have stalled. Objective: This study aimed to assess the effect of the COVID-19 immunisation on semen parameters in comparison with unvaccinated males. Patients and Methods: Semen analysis was used to survey adult males in Egypt between March and October 2022 before and 3 and 6 months after immunisation. Results: Regardless of immunisation status or prior COVID-19 medical history, 220 men who are now above the age of 18 and reside in Upper Egypt are eligible to participate in the survey. Respondents have to be at least 18 years old and have never had main or secondary infertility identified by a doctor. The results of the multivariable adjusted analysis showed that semen parameter affliction was not linked to COVID-19 immunisation. Conclusion: Overall, this cross-sectional study revealed that males 18 years of age and older did not have an elevated risk of alteration of semen parameters following COVID-19 immunisation.