Background: The student-instructor relationship as well as effective learning strategies is an important component of an academic program that magnifies the feeling of trust and respect between the students and instructor. Therefore, evaluation of the student-instructor relationship provides crucial understanding to reach a good academic environment. Objective: The study aims at assessing the students - instructor relationship within the educational environment and evaluating the emotional perception toward relationships among nursing students and their instructors. Subjects and Methods: The study was conducted on Nursing Collegians of Morning and Evening Studies at the Nursing Faculty, at Baghdad University.The assessment approach was used over six months (from January 2018 to June 2018) to achieve the study objectives. A sample of 200 Nursing Collegians was recruited. Student-Instructor Scale was adopted and used for the current study. The scale consisted of 36 items. The scale defines two domains regarding instructor relationships; the first domain is regarding the connectedness with an instructor, and the second domain of the scale is concerned with instructor anxiety. Results: The students show higher scores of instructor connectedness and anxiety (89% and 83%) which means they perceive a strong feeling of connectedness with an instructor and show generalized anxiety regarding this relationship at the same time. Conclusions: There is a strong positive relationship between students and instructors in nursing education, and this positive relationship is an important moderator for improving the student's academic performance.