Background: Medicinal plants are a generous source of our drugs and remedies, coming from their fabulous secondary metabolites, which involve different classes such as alkaloids, glycosides, essential oils, phenolic compounds, and steroids. Callistemon viminalis is a small tree cultivated in Iraq, that owns very interesting bioactive compounds especially, essential oils, phenolic derivatives, and terpenoids. Objective: To investigate the bioactive compounds from the aerial part of the Iraqi Callistemon viminalis plant. Material and methods: The plant was extracted by Soxhlet apparatus and fractionated with solvents that differ in polarity, the n-butanol and ethyl acetate fractions were applied to Liquid Chromatography-Tandem mass spectrometer, while the hexane defatting extract was applied to Gas Chromatography-Mass spectrometer analysis to discover to components of the plant. Results: The results show the presence of many glycosides, flavonoids, terpenoids, and alkaloids like Kaempferol, Astragalin, Quercitrin, Scutellarin alpha-Phellandrene, 3-Carene, (-)-Spathulenol and, Dihydroepinatalensine. Conclusion: Callistemon viminalis aerial part involves diverse types of important that own important medical activity, they are mostly flavonoids, phenolic derivatives, terpenoids, terpenes, and glycosides. Also, the study supports the presence of powerful anti-inflammatory flavonoids and terpenoids that are involved in the traditional use of the plant in the relief of tissue inflammations.