Background: PGF2α-PMSG is one of the hormones used to increase production and reproduction, but there are some side effects regarding its use and the length of its effect. Thus, improvement of the action of hormones and reduction of their side effects by reformulation and optimization of PGF2α-PMSG based on the nano-lipid delivery system became the target. Objective: This study aimed to reformulate and improve PGF2α-PMSG based on the nano-lipid delivery system.  Materials and methods: In this study, Nano-Lipid Carriers (NLCs) contained fatty acids to obtain nanoparticles with small particle sizes. The hormone Nano lipid was prepared by solvent diffusion method, and has the characters and properties of Nano-lipid. The results: The results were optimized nanoparticle has a size of Nano-lipid Carrying PGF2α  range between 50-166.6 nm and Nano-lipid Carrying PMSG  85.7-300 nm. The entrapment efficiency of hormone SNL PMSG was 79.04 ± 4.96 and SNL PgF2α was 84.11 ± 5.55. The results of characterization for the SLNs under transmission electron microscopy were generally round and uniform in shape. FTIR and XRD analyzers indicate that the hormone is properly laden within the amorphous nanostructure. It also proved that Nano-lipid formula has high stability at pH and Osmo-tolerance. The Nano-Lipid structure PGF2α and PMSG formulas were represented ʎ max 450 nm in UV-visible. Conclusion: The present study showed that the Nanostructured -Lipid Carriers had the ability in producing high-efficiency of PGF2α and PMSG hormones.