Background: The evidence has shown that Silver nanoparticles (AgNPs) are increasingly used in various fields, including medical, food, health care, consumer, and industrial purposes due to their distinctive physical and chemical properties. Despite the advantages of NPs, a number of problems have raised the possibility of toxicity in both people and animals. Objective: The primary aim of the current study is to assess the impact of AgNPs synthesized by green method from plant extract Borago officinalis on reproductive system. Material and methods: AgNPs synthesized by using Borago officinalis flower extracts as reducing agents/stabilizers which be a friendly technique to environment. A total of 40 adult male rats are taken and divided to 4 equal groups: Group 1 treated with distilled water, Group 2 received orally only plant extract (5mg/kg), and Groups 3 and 4 injected through intraperitoneal cavity with AgNPs 30 and 50 mg/kg respectively. We measured oxidative parameters of malondialdehyde (MDA) levels and blood testosterone hormone levels after 35 days. Results: Our results demonstrated that AgNPs rise significantly the MDA value in the animal groups that treated with different concentrations of AgNPs in comparison to the control groups which treated only with Borage extract. Also, a non-significant decrease of testosterone levels in animals treated with Borage extract in contrast to control group which have a significant decrease in testosterone which treated with different concentrations of AgNPs. Conclusion: The Borage flower crude extract are nontoxic, where AgNPs (even if it was produced from plant extract) have a negative impact on male reproductive system by alteration the testicular functions especially the testosterone.