Background: Hepatitis B virus (HBV) infection is a well-recognized cause of liver disease and represents a major problem for global health. Understanding factors associated with HBV transmission could offer practical applications to reduce disease risk. Objective: The aim of the current study to assess prevalence and risk factors of HBV infection in city of Erbil during 2020 and 2021. Patients and methods: HBV infection data were collected for all registered patients, including those who had chronic bleeding disorder (hemophilia) from the Center of Infectious Disease Control and Nanakaly Hospital for Hematology & Oncology in Erbil. A structured data collection form was used to abstract data about route of infections and patients' demography. Results: The overall HBV infection rate in 2021 was significantly higher than in 2020, but it was particularly high among hemophilia patients (7.34%). Vast majority of the infections were found to be caused by visiting dental clinics. Occupation significantly varied among HBV-positive individuals, and free workers had a higher risk of the infection than others. The infection rate was significantly higher among males than females. The rate of family members of infected individuals who received the HBV vaccine was significantly lesser than those who did not receive the vaccine. Conclusion: Despite the availability of effective vaccines to the public, the risk of HBV infection is continuously growing with the advances in population dynamics in the city.