Background: Breast cancer is the most prevalent kind of female cancer among women today affects both emerging and industrialized nations. Objective: This study intended to assess the levels of Visfatin and pro-inflammatory cytokines (TNF-α) in Iraqi women who had breast cancer. Patients and methods: In this study, serum concentrations of Visfatin and TNF-α were determined using samples from 90 Iraqi women (50 patients and 40 healthy volunteers). Results: The current study's findings demonstrated that both Visfatin and TNF-α were highly elevated in patients with breast cancer (P>0.05), and they were also strongly associated. Conclusions: This study has found an association between increased levels of serum Visfatin and TNF-α and breast cancer.