Background: The oxidative stress is imbalance between antioxidants and pro-oxidants. MDA is product of lipid peroxidation, which increases due to increased oxidative stress that can be induced by iron. Vitamin C acts as antioxidant. Objective: The goal of our study is to verify the association between MDA level and vitamin C. Subjects and Method: 200 volunteers were participated in this study, 121 of them were supplemented with ferrous sulphate capsule and were considered as case group and 79 participants didn't take iron preparation and were considered as control group. The case group of 121 individuals were undergo further subgroup ,they were include, firstly 80 subjects were administered with iron, without vit C. Out of them, we took only 43 participants to compare with the second subgroup: 41 subject administered iron plus vit C.MDA, S. iron, vitamin (vit) C were measured. Results: Biochemical data showed significant higher level for malondialdehyde (MDA) and serum iron in supplemented group compared with non-supplemented group. Also, the results revealed higher level, but non-significant, of serum iron, significant lower MDA level and significant higher level for vit C in subgroup supplied with vit C plus iron compared with the other subgroup. Conclusion: Vit C has ab antioxidant effect through decrement of MDA level that results from lipid peroxidation, which is enhanced by iron.