Introduction: Toxoplasma gondii is a protozoan parasite that lives intracellular and has been linked to autoimmune disease. It also can modulate immune responses to varying degrees. Rheumatoid arthritis, sometimes known simply as RA, is a prevalent autoimmune disease that is distinguished by its multiple pathogenic pathways, which ultimately lead to persistent inflammation and damage to the joints. MicroRNAs play a crucial role in immune response regulation and can be used as novel biomarkers to improve diagnosis, treatment, and disease states.  Objective:  The purpose of this study was to investigate the levels of expression of the microRNAs miR-146a and miR-155 as potential biomarkers for the diagnosis of rheumatoid arthritis (RA) in individuals who were infected with toxoplasmosis. Patients and Methods: The study was conducted on 50 Rheumatoid Arthritis patients positive for toxoplasmosis as diagnosed by ELISA (divided into two groups according to treatment response) and 50 age and sex-matched healthy subjects as a control group. Purified RNA was done, then real-time PCR was carried out using specific primers for MicroRNA-146a and MiR-155. Results: A significant up-regulation in both Micro-RNA in the studied groups when compared with the control group. A high level of miR-146 expression was associated with low expression for miR-155 observed in the negative response group for treatment when compared with the positive response group.  Conclusion: the miR-146 and mir-155 could be used as biomarkers for host response during the biological treatment.