Background: A highly helpful diagnostic technique for determining the cause of shoulder pain is magnetic resonance imaging (MRI). Objective: To study the role of MRI in the diagnosis of chronic non-traumatic shoulder pain. Subjects and methods: In this cross-sectional observational research, 60 patients (32 males and 28 females) presented with chronic shoulder pain and limitation of movement. All cases were recruited from the Orthopedic Surgery and Radiodiagnosis Departments at Zagazig University Hospitals. All cases were assessed by Magnetic Resonance Imaging. Results: Fifty-three cases had rotator cuff pathologies, thirty-seven cases had acromioclavicular osteoarthritis, twenty-nine cases had bursitis, fifteen cases had biceps pathologies and nine cases had miscellaneous conditions. A study; 16 (35%) Sub coracoid, 10(16.7%) in Sub-acromial sub deltoid and (5%) Subscapular. Among the current study, 9 (15%) of cases were miscellaneous. Four cases were suffering from interosseous cystic lesions (6.7%) of all cases. Lipoma was seen in 3(5%) of cases. Ganglion cyst was seen in 2(3.3%) of cases. Conclusion: MRI of the shoulders aided in understanding different underlying pathology of chronic shoulder pain and revealed that dependence on only clinical findings and diagnosis could delay their management as well as chronicity development.