Background: Nephrotic syndrome (NS) is one of the most widespread chronic renal illnesses in childhood. Homocysteine (Hcys) metabolism uses vitamin B12 and folic acid as a cofactor. Objective: The current study investigated the probableassociationamongvitamin B12 and folic acid with nephrotic patients in various stages comparing to healthy ones among Egyptian children. Patients and methods: The current researchwasdone on 60 patients in relapse and remission compared to 30 healthy children.Folic acid, vitamin B12, albumin and cholesterol in serum were measured in all patients and controls. Results: Level of vitamin B12 was significantly lower in relapse than controls. Mean vitamin B12 was significantly higher in relapse group than in the remission one. Vitamin B12 was significantly lower in remission group than in the control group. No majorvariation was found among patients in relapse and controls as regard serum folic acid level. No significant alteration was found amongst patients in remission and relapse as regard serum folic acid level. Same results were found between patients in remission and control as regard to folic acid. Conclusions: Decreased levels of B12 were associated significantly with NS in relapse. Understanding the correlation of vitamin B12 and folate supplementation in childrenwith NS mightputphysicians and scientists in preferable situation to create informed remediation policy and decisions.