Background: Breast cancer is the most common occurring cancer in women worldwide. To guide current breast cancer screening program, the level of knowledge about breast cancer should be evaluated .A high level of knowledge and practice among female health care providers regarding risk factors for breast cancer and screening methods are important in increasing awareness in the general population.  Objective: This study aims to assess perception of female health care providers regarding breast cancer at El-Minia oncology center.: Design: Descriptive research design was used for conducting the study. Sample: A convenience sample was used to choose 148 female health care providers. Setting: The study was carried out at oncology center at Minia city, Egypt. Tools: It consisted of 4 parts: part I: Concerning with demographic characteristics of female health care providers. Part II: female health care providers' knowledge questionnaire. Part III: female health care providers᾿ attitude toward breast cancer. Tool VI: female health care providers' reported practice regarding breast self-examination. Results: The study result revealed that, 89.9% of female health care providers have satisfactory levels of total knowledge regarding breast cancer. 98.6% of female health care providers have positive attitude toward breast cancer and 97.3% of female health care providers have adequate level of reported practice. Conclusion: there's highly statistically significant between female health care providers' total perceived breast cancer include total knowledge, total practice and attitude toward breast cancer. Recommendations: continuous education program to improve knowledge and practice of female health care providers through carrying out continuing educational programs about how to prevent breast cancer with other organizations and institutions.