Background: Antibiotic resistance is a serious public health concern that is becoming worse. Although several new antibiotics have been created in recent years, none have demonstrated enhanced efficacy against bacteria that are multidrug resistant. Objective: Long recognization for the all-encompassing antibacterial properties of Cu/CuO NPs. Subjects and Methods:  FT-IR spectroscopy, UV-visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy were used to analyze the produced nanoparticles (SEM). Results: The UV-visible spectroscopy revealed a Surface Plasmon Resonance (SPR) at 355 nm which is characteristic of silver nanoparticles. FT-IR spectral analysis showed the functional groups which were responsible for the reduction of AgNPs and capping and stabilization. The antimicrobial assay confirmed good inhibitory activity against Streptococcus mutans. Conclusion: The outcomes demonstrated that the addition of Cu/CuO NPs to medications had considerable antibacterial effects.