Introduction: The development of nanotechnology, due to the unique physical and chemical features of nanomaterials. Nanomaterial therapy for RA can increase bioavailability and target damaged joint tissue Objective: This study aimed to check the effect of gold nanoparticles (AuNPs) with resveratrol by polyethylene glycol to manage the levels of ten immune markers of rheumatoid arthritis patients. Patients and Methods: Gold nanoparticles (AuNPs) were functionalized with resveratrol by polyethylene glycol and utilized to manage the levels of 10 immune markers of 84 rheumatoid arthritis patients and 20 healthy volunteers ranging in age from 18 to 80 years. Results: The synthesized gold nanoparticles, before and after functionalization, were characterized by UV-Vis spectrophotometer, Fourier-transforms spectroscopy, zeta potential, and FE-SEM. The levels of 10 immune markers in the sera of RA patients (IgA, IgE, IgG, RF, ANA, anti-dsDNA, C3, C4, IL-6, and IL-33) were tested by ELISA kits to study the in vitro effects of treatment with functionalized and non-functionalized AuNPs on their immune response. Conclusion: The levels of all markers were decreased significantly in RA patients after exposure to AuNPs or AuNPs-PEG-Res. However, the levels of IgE, RF, ANA, dsDNA, C3, C4, and IL-33 showed significantly higher reduction in the presence of AuNPs as compared to AuNPs-PEG-Res, whereas the opposite was observed for the levels of IgA, IgG, and IL-6.