Background: The intensive care unit (ICU) plays a crucial role in managing and treating some of the most complex and serious disorders that affect the human body. Patients and Methods: A total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted to the ICU. in this study admitted to the intensive care units (ICUs) of Alzahraa and Alkarama hospitals for more than 48 hours between 3^rd  October 2021 to 20^th February 2022. Each patient's name, age, gender, underlying clinical condition, ICU admission date, indoor admission date, previous antibiotic intake history, current ICU therapy, and clinical outcome were recorded. ‎ Results: Thirty (36.5%) female and‏‎ 52 (63.5%) male patients were admitted to the ICU. They were distributed 69 (84.1%) gram-‎negative bacteria. Gram positive 13 (15.9%). Nitrofurantion ‎was most active against E. coli. Piperacillin-‎tazobactam was most active against ‎Proteus mirabilis. AmpC ß-lactamase was detected by phenotypic and genotypic ‎procedures, phenotypically AmpC producers for klebsiella pneumoniae ‎‎13 (68%). Genotypically isolates bla_EBC 4 (30.7%), bla_CIT ‎‎13 (53.8%). However, phenotypically survey of AmpC pseudomonas aeruginosa was ‎‎9 (45%). Genotypically isolates bla_EBC was 6 (50%). Phenotypically survey of AmpC producers for E. coli was 9 (52%) and ‎genotypically isolates bla_EBC 3 (30%), bla_CIT 1 (10%). While, DHA, ‎MOX ACC, FOX genes were absent among all isolates. Conclusion: klebsiella pneumoniae showedAmpC ß-lactamase comprised(bla_EBC andbla_CIT. while no isolate have DHA, MOX ACC, FOX genes). As for E.Coli AmpC ß- lactamase comprised (bla_EBC andbla_CIT, while no isolates have AmpC DHA, MOX ACC and FOX.